ykzR [2018-10-11 18:13:31]

unknown

locus: BSU13799

pI: 9.85

mw: 9.40 kDa

protein length: 83 aa

gene length: 249 bp

function: unknown

product: unknown

essential: -

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 1,446,317 → 1,446,568

The protein

Structure

• [PDB|4S3K] (from B. megaterium, 31% identity)

additional information

• Although related to the glycosyl hydrolase 18 family, lacks the conserved Glu active site at position 30, which is relaced by a Lys residue, suggesting it has no glycosidase activity (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|FC4C5377EF1D794DCB19D0A8AFE958B84BD9C8BE|ykvP]-[gene|E8311435A86835799E9093DD9CF3733CFFF18DCC|ykzQ]-[gene|BC8CBFF0C2F3C2BEE3275E2CB65D41F2AFC0A1CD|ykvQ]-[gene|4FDAD5E5660EDB346A37C1F41BD8B7B66561E529|ykzR]
  description: [Pubmed|11011148,22383849]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|11011148,15699190], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]) [Pubmed|11011148,15699190]

Biological materials

Mutant

• BKE13799 (Δ[gene|4FDAD5E5660EDB346A37C1F41BD8B7B66561E529|ykzR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13799 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTGGTGTCAAAAATAATTG, downstream forward: _UP4_TAATGATTTATAGTTCAGAA
• BKK13799 (Δ[gene|4FDAD5E5660EDB346A37C1F41BD8B7B66561E529|ykzR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13799 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTGGTGTCAAAAATAATTG, downstream forward: _UP4_TAATGATTTATAGTTCAGAA